The Sanctuary of medieval origins, represents one of the most important symbols of the inhabitants of Tolfa. To reach the Church it is necessary to travel the suggestive uphill road, surrounded by the pretty gardens of the surrounding houses. The Church has reached the present day after numerous restorations. The facade opens onto a square embellished by a large single-flight staircase. The structure of the bell tower made of brickwork of a typical reddish color, stands out next to the mass of the Church in white and pink limestone. The portal is surmounted by a very simple rose window. Inside, the sanctuary houses the precious canvas painted in oil at the end of the 16th century depicting the deposition of the cross with the Virgin Mother seated, who holds in her bosom the body of her son just taken down from the Cross. The work is attributed to the Carracci school. The imposing marble altar, however, was designed by the Franciscan artist Father Cosma da Cori, under the guidance of a lay hermit, Fra' Giuseppe Catalini, who dedicated fifty years of his life (1861-1911) to the care of the Sanctuary.